Journal: Obesity Medicine

Article Title: Relationship between microRNA expression profiling and gestational diabetes: A systematic review

doi: 10.1016/j.obmed.2023.100487

Figure Lengend Snippet: Fig. 2. HeatMap and cluster patterns from gene expression pathways affected by miRNAs. A: Target of upregulated miRNAs in placental tissue, placenta-derived cells (Mononuclear cells), peripheral blood, plasma, plasma Evs, and serum B: Target of downregulated miRNAs in placental tissue and placenta-derived exosomes.

Article Snippet: RT-qPCR U6 n/a n/a HUANG et al., 2021* Trizol n/a RT-qPCR GAPDH n/a n/a Li 2015ɨ** RecoverAllTM Total Nucleic Acid Isolation kit (Ambion) y TRNzol reagent (TIANGEN Biotech, Beijing, China) miRNA first-strand cDNA kit (TIANGEN Biotech, Beijing, China) miRcute miRNA qPCR detection kit (TIANGEN Biotech, Beijing, China) U6 Agilent Human miRNA Microarray (Agilent) n/a Li et al., 2018** KingFisher Automated Purification Kits (Thermo Fisher) miRCURY LNATM microRNA Array Hi-Power Labeling kit (Qiagen) n/a Hairpin-itTMmiRNAs RealTime PCR Quantization Kit (GenePharma) n/a n/a n/a Li W 2022* TRIzol reagent (Magen Biotechnology Co.) TaKaRa PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) miScript SYBR Green PCR Kit (TaKaRa) U6 n/a n/a Peng et al., 2019* Trizol (Ambion, USA) miRcute miRNA FirstStrand cDNA Synthesis Kit (TianGen, Beijing, China) miRcute miRNA qPCR Detection Kit (SYBR Green) (TianGen, Beijing China) U6 n/a n/a Sun et al., 2020* Trizol reagent (Invitrogen, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan Univel PCR Master Mix (Applied Biosystems, Foster City, CA, USA) U6 n/a n/a Tang 2020ᵻ* Trizol Reagent (Invitrogen, Carlsbad, CA, USA) M-MLV Reverse Transcriptase (Invitrogen) qRT–PCR (BIONEER, Daejeon, Korea) U6 n/a NEBNext Multiplex Small RNA Library Prep Set for Illumina_x0003_ (NEB, Ipswich, MA, USA) Wang S 2020* Trizol reagent (Invitrogen) Transcriptase Kit (Takara, Otsu, Japan) SYBR Green PCR Master Mix (Takara) U6 n/a n/a Zhang Lu 2021* Trizol n/a RT-qPCR U6 n/a n/a Zhao et al., 2014* Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription kit TaqMan microRNA assay (Applied Biosystems, Branchburg, NJ, USA) U6 n/a n/a Serum Abdeltawab et al., 2020** miRVana PARIS kits (Life Technologies, Grand Island, NY, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan quantitative RTPCR.

Techniques: Gene Expression, Derivative Assay, Clinical Proteomics